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The initial preprocessing steps for the bead by gene expression data are similar to other spatial Seurat analyses and to typical scRNA-seq experiments. Here, we note that many beads contain particularly low UMI counts but choose to keep all detected beads for downstream analysis.
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PhD Student

Interviewed at Duke University

4.3★
Aug 13, 2021

The initial preprocessing steps for the bead by gene expression data are similar to other spatial Seurat analyses and to typical scRNA-seq experiments. Here, we note that many beads contain particularly low UMI counts but choose to keep all detected beads for downstream analysis.

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